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KMID : 0380620150470040438
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 4 p.438 ~ p.445
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Optimization of γ-Aminobutyric Acid (GABA) Production Using Immobilized Lactobacillus plantarum K154 in Submerged Culture of Ceriporia lacerata
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Lee Eun-Ji
Lee Sam-Pin
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Abstract
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The production of GABA was optimized by co-cultivation of immobilized Lactobacillus plantarum K154 (ILK) with Ceriporia lacerata cultures. The mycelial culture of C. lacerata was performed in a defined medium containing 3% glucose, 3% soybean flour, and 0.15% MgSO4 in a submerged condition for 7 days at 25°C, resulting in the production of 29.7 g/L mycelia, 3.1 g/L exopolysaccharides, 2% (w/w) β-glucan, 68.96 unit/mL protease, and 10.37 unit/mL α- amylase. ILK in C. lacerata culture showed viable cell counts of 3.13×109 CFU/mL for immobilized cells and 1.48×108 CFU/mL for free cells after 1 day. GABA production in the free and immobilized cells was 9.96 mg/mL and 6.30 mg/ mL, respectively, after 7 days. A recycling test of ILK in the co-fermentation was consequently performed five times at 30°C for 15 days, resulting in the highest production of GABA. GABA could also be efficiently overproduced by cocultivation with the produced polysaccharides, β-glucan, peptides, and probiotics.
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KEYWORD
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Ca-alginate bead, immobilization, γ-aminobutyric acid, Lactobacillus plantarum, Ceriporia lacerata
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